The factors affecting the performance of large flanges
In the consumption of large flanges, there are many factors affecting the performance of large flanges. Let’s talk about several common factors below. First, the annealing temperature (annealing temperature is the temperature parameter when primers and templates are separated, and when 50% of primers and complementary sequences are double-stranded DNA molecules, it is an important factor affecting the specificity of PCR. Ideally, the annealing temperature is low enough to ensure the effective annealing of homologous primers and high enough to reduce non-specific separation. Reasonable annealing temperature ranges from 55 C to 70 C. The annealing temperature is generally set at 5 C lower than that of the primer Tm.
Can we reach the regular temperature? Large flanges are usually treated by solid solution heat treatment, which is commonly known as “annealing”,and the temperature range is 1040 ~ 1120 (“Japan Specification”). You can also inspect the annealing furnace and see that the large flanged pipe fittings in the annealing zone should be incandescent, but not softening and sagging.
Secondly, the sealability of the furnace body (the shell is welded by steel plate and section steel, the trolley is welded by section steel and steel plate, and the trolley reduces the heat radiation and convection loss through the soft contact and sand sealing mechanism with the furnace lining, thus effectively ensuring the sealability of the furnace body). Bright annealing furnaces should be sealed off from the outside air; hydrogen is used as maintenance gas, as long as one exhaust port is open (used to ignite the exhausted hydrogen). The method of inspection can be used soap water to wipe the gap of each joint of annealing furnace to see if it can run off. The most easily run-off center is the center of the annealing furnace’s inlet pipe and outlet pipe. This central sealing ring is particularly easy to wear and tear, so it should be checked and replaced frequently.
Secondly, the maintenance pressure, in order to avoid slight leakage, the furnace maintenance gas should adhere to a certain positive pressure, if it is hydrogen maintenance gas, the general request is more than 20 kBar. Annealing atmosphere: Pure hydrogen is usually used as annealing atmosphere, the purity of the atmosphere is better than 99.99%. If the other part of the atmosphere is inert gas, the purity can be lower, but absolutely can not contain excessive oxygen, water vapor.